CLU elisa kit product blog
Tags: ELISA Kit; CLU elisa kit; CLU; Clusterin (Apolipoprotein J);
The Rat CLU clu (Catalog #MBS564106) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS564106 ELISA Kit recognizes Rat CLU.The CLU clu product has the following accession number(s) (GI #42740907) (NCBI Accession #NP_976084.1) (Uniprot Accession #P10909). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the CLUSTERIN present in samples reacts with the anti-CLUSTERIN antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-CLUSTERIN antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound CLUSTERIN. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3\',5,5\'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CLUSTERIN in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CLUSTERIN in the test sample. The quantity of CLUSTERIN in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Samples: Immunoperoxidase Assay for Determination of CLUSTERIN in Rat Samples
Assay Type: Sandwich. Intended Uses: The CLUSTERIN test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring CLUSTERIN in biological samples of Rat. Rat Clusterin ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. CLU also interacts with the following gene(s): APP, CLUAP1, MMP25, PON1, PRNP. The following patways have been known to be associated with this gene. Blood, Brain, Eye, Kidney, Liver, Muscle, Nerve, Prostate, Testis, Vascular tissues are correlated with this protein. Adenocarcinoma, Alzheimer Disease, Breast Neoplasms, Cardiovascular Diseases, Drug Toxicity, Inflammation, Kidney Diseases, Neoplasms, Neurodegenerative Diseases, Prostatic Neoplasms are some of the diseases may be linked to Rat Clusterin ELISA Kit.