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CK-18 elisa kit product blog

Posted on 2014-07-18 19:29:19 by mybiosource_staff
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Tags: ELISA Kit; Human ELISA Kit; cytokeratin 18 (CK-18); CK-18 elisa kit; CK-18;
The Human CK-18 krt18 (Catalog #MBS261909) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS261909 ELISA Kit recognizes Human (General) CK-18.

The CK-18 krt18 product has the following accession number(s) (GI #34037) (NCBI Accession #CAA31375.1) (Uniprot Accession #P05783). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human cytokeratin 18 (CK-18) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) CK-18.

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human CK-18 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant CK-18 concentration
Assay Type: Sandwich
Detection Range: 10 ng/ml-0.156 ng/ml
Sensitivity: 0.05 ng/ml. Intra-assay Precision: <= 8%
Inter-assay Precision: <= 12%. Human cytokeratin 18 (CK-18) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. CK-18 also interacts with the following gene(s): CASP6, KRT8, TCHP. The following patways have been known to be associated with this gene. Adenocarcinoma, Breast Neoplasms, Carcinoma, Fatty Liver, Fibrosis, Liver Cirrhosis, Liver Diseases, Liver Neoplasms, Neoplasm Metastasis, Neoplasms are some of the diseases may be linked to Human cytokeratin 18 (CK-18) ELISA Kit. Blood, Eye, Kidney, Liver, Lymph, Lymph Node, Muscle, Prostate, Stomach, Vascular tissues are correlated with this protein.
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