Chicken IgY elisa kit product blog
Tags: ELISA Kit; Chicken IgY; Chicken IgY elisa kit;
The Chicken Chicken IgY n/a (Catalog #MBS564008) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS564008 ELISA Kit recognizes Chicken Chicken IgY.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: The IgY test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgY in chicken biological samples. If the ELISA is to be used outside the intended use, the user may need to optimize for said use.
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the IgY present in samples reacts with the anti-IgY antibodies, which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgY antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgY. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3\',5,5\'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgY in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgY in the test sample. The quantity of IgY in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Samples: Chicken biological samples
Assay Type: Sandwich. Chicken IgY ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.