chGH recombinant protein product blog
Tags: Recombinant Protein; chGH recombinant protein; chGH; growth hormone mutein;
The chGH gh1 (Catalog #MBS400013) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase.The chGH gh1 product has the following accession number(s) (GI #126352373) (NCBI Accession #NP_001075417.1) (Uniprot Accession #P01245). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
The sequence of the first five N-terminal amino acids of chicken recombinan t growth hormone mutant was determined and was found to be Ala-Thr-Ph e-Pro-Ala.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including growth hormone mutein are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.
Solubility: It is recommended to reconstitute the lyophilized chicken recombinant growth hormone mutant in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml and not more than 3 mg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Protein Content: Protein quantitation of chicken recombinant growth hormone mutant was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.74 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0 This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Dimers and Aggregates: The purified chicken recombinant growth hormone G119R (22K) consists of > 99% monomers as determined by gel-filtration chromatography. Endotoxin: Less than 0.1 ng/ug (IEU/ug) of chicken recombinant growth hormone G119R (22 K).
Formulation: The chicken recombinant growth hormone was lyophilized from a concentrated (1mg/ml) solution with 0.3% NaHCO3 adjusted to pH 8.
Biological Activity: Chicken recombinant growth hormone G119R mutant did not bind to ovine growth hormone extracellular domain (ECD) and was devoid of any biological activity in FDC-P1 3B9 cells. However, in binding experiments that were carried out using chicken liver membranes, both ovine growth hormone and chicken recombinant growth hormone showed similar IC50 values in competition with 125I-oGH, while the IC50 of chicken recombinant chicken growth hormone G119R mutein was 10-fold higher. These results emphasize the importance of species specificity and indicate the possibility of antagonistic activity of chicken recombinant growth hormone G119R in homologous system. The following patways have been known to be associated with this gene. chGH also interacts with the following gene(s): GHR, IL9R, JAK2, JAK3, PRLR. Breast Neoplasms, Cardiovascular Diseases, Growth Disorders, Insulin Resistance, Isolated Growth Hormone Deficiency, Type II, Liver Diseases, Nervous System Diseases, Obesity, Pituitary Neoplasms, Weight Gain are some of the diseases may be linked to Chicken growth hormone mutein (G119R).