Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Blog  rightarrow CFLAR elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • anti-FPR2 antibody blog
 • ARMCX1 blocking peptide blog
 • IgG secondary antibody blog
 • TMCC1 sirna blog
 • RNASE3 sirna blog
 • SPARC native protein blog
 • pGM CSF recombinant protein blog
 • anti-CORO2B antibody blog
 • Rabbit anti Mouse IgE (Fc specific) secondary antibody blog
 • KRTAP10-1 sirna blog
 • MLT elisa kit blog
 • anti-CD59 antibody blog
 • GLOD4 sirna blog
 • TRAF6 blocking peptide blog
 • Hepatitis C Virus (HCV) Core Antigen (a.a. 2-120) genotype 1, Recomb. recombinant protein blog
 • Bence Jones Kappa Light Chain native protein blog
 • SYT9 sirna blog
 • ZNF625 sirna blog
 • IgG secondary antibody blog
 • MBP elisa kit blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • Glucagon (GC) ELISA Kit
 • APCS cDNA Clone
 • CENPH Antibody
 • Carbonic Anhydrase III (CA3) Antibody
 • GrpE Recombinant Protein
 • TFAM cDNA Clone
 • lactoferrin (LTF) Native Protein
 • Macrophage inflammatory protein-3alpha (MIP-3alpha) ELISA Kit
 • CD32b (pY292) (FCGR2B) Antibody
 • Plexin-A1 (PLXNA1) Recombinant Protein
 • ARHGEF11 Antibody
 • Paraoxonase (PON) ELISA Kit
 • Intelectin 1 (ITLN1) ELISA Kit
 • Goat anti Human IgM (Fc specific) Secondary Antibody
 downarrow more ...

CFLAR elisa kit product blog

Posted on 2013-12-08 04:08:19 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Human ELISA Kit; CFLAR; CASP8 and FADD like apoptosis regulator (CFLAR); CFLAR elisa kit;
The Human CFLAR cflar (Catalog #MBS7230831) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS7230831 ELISA Kit recognizes Human (General) CFLAR.

The CFLAR cflar product has the following accession number(s) (GI #12643547) (NCBI Accession #O15519.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human CASP8 and FADD like apoptosis regulator (CFLAR) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) CFLAR.

Principle of the assay: CFLAR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CFLAR antibody and an CFLAR-HRP conjugate. The assay sample and buffer are incubated together with CFLAR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CFLAR concentration since CFLAR from samples and CFLAR-HRP conjugate compete for the anti-CFLAR antibody binding site. Since the number of sites is limited, as more sites are occupied by CFLAR from the sample, fewer sites are left to bind CFLAR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CFLAR concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 0.5-10ng/mL
Sensitivity: 0.1ng/mL. Intended Uses: This CFLAR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CFLAR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Human CASP8 and FADD like apoptosis regulator (CFLAR) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Adenocarcinoma, Breast Neoplasms, Disease Models, Animal, Inflammation, Leukemia, Liver Diseases, Lung Neoplasms, Necrosis, Neoplasms, Experimental, Nervous System Diseases are some of the diseases may be linked to Human CASP8 and FADD like apoptosis regulator (CFLAR) ELISA Kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions