Cd28 secondary antibody product blog
Tags: Secondary Antibody; CD28; Cd28 secondary antibody;
The Cd28 n/a (Catalog #MBS673299) is an Antibody produced from Syrian Hamster and is intended for research purposes only. The product is available for immediate purchase. The Hamster Anti-Mouse CD28-BIOT reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s CD28 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry.Flow Cytometry � Quality tested1,2
Immunoprecipitation � Reported in literature 1
Costimulation � Reported in literature 1-3,7-9,11
Stimulation � Reported in literature 10
Activation � Reported in literature 5
Blocking � Reported in literature 6
Flow Cytometry:
Biot conjugates: < = 2ug/106 cells
Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need. Researchers should empirically determine the suitability of the Cd28 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Cd28 n/a product has the following accession number(s) (GI #192485) (Uniprot Accession #P31041). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Hamster Anti-Mouse CD28-BIOT with the following immunoassay(s):
Testing Data (TD) (BALC/c mouse thymocytes were stained with Hamster Anti-Mouse CD28-UNLB (MBS673297) followed by Goat Anti-Hamster IgG (H+L) Mouse/Rat ads-PE (MBS674823))
CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T lymphocytes and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) on B cells and other antigen presenting cells and plays an important role in the interaction between T cells and B cells. CD28 is a costimulatory receptor involved in many, but not all, T-cell independent immune responses.
Immunogen: C57BL/6N mouse T cell lymphoma EL-4 cell line. Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Cd28 are readily searchable from our website. Different antibodies against the same target such as Cd28 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.