Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow CD25 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • TRERF1 sirna blog
 • IFNAR1 shrna blog
 • CMTM7 shrna blog
 • PEA15 blocking peptide blog
 • ANPCE elisa kit blog
 • anti-Staphylococcus aureus, Enterotoxin I antibody blog
 • Ureido Chemotactic Peptide [Benzylureido-Met-Leu-Phe] peptide blog
 • ANG1 elisa kit blog
 • Mouse Anti-Human IgA2 (alpha 2 chain specific) secondary antibody blog
 • glutamate elisa kit blog
 • HTR7 blocking peptide blog
 • anti-AR antibody blog
 • m-dPEG8-DSPE reagent blog
 • HEATR4 sirna blog
 • anti-Clostridium tetani, Tetanus toxin (tetanospasmin, TeNT) antibody blog
 • SMPD2 blocking peptide blog
 • TSPAN12 shrna blog
 • SIRT1 blocking peptide blog
 • PPP1R13L sirna blog
 • HLX sirna blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Osteocalcin (OT) ELISA Kit
 • ABCA8 Antibody
 • CHAT Antibody
 • LHCGR Peptide
 • Rhinovirus outer capsid Protein 3 (VP3) Antibody
 • Vascular Endothelial Growth Factor (VEGF) ELISA Kit
 • Kinesin Family, Member 1A (KIF1A) ELISA Kit
 • Granulocyte Macrophage-Colony Stimulating Factor (GM) Recombinant Protein
 • collectin sub-family member 12 (COLEC12) ELISA Kit
 • Mammal Tissue Protein Extraction Reagent Western Blot Related Reagent
 • Cathepsin K (CTSK) Antibody
 • Hepatitis B Surface Antigen ad,ay Antibody
 • CD106 Antibody
 • ABCB10 Antibody
 downarrow more ...
separator

CD25 elisa kit product blog

separator
Posted on 2013-10-13 09:44:53 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; CD25; Human; No cross reactivity with other human soluble receptors ELISA Kit; CD25 / IL-2R; CD25 elisa kit;
separator
The Human. No cross reactivity with other human soluble receptors. CD25 pmp22 (Catalog #MBS335497) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS335497 ELISA Kit recognizes Human. No cross reactivity with other human soluble receptors. CD25.

The CD25 pmp22 product has the following accession number(s) (GI #55904) (NCBI Accession #CAA44297.1) (Uniprot Accession #P25094). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

MyBioSource ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents, and a detailed procedure.

CD25 pmp22

Samples: Supernatants, buffered solutions or serum and plasma samples
Sensitivity: The sensitivity or minimum detectable dose of CD25 using this CD25 ELISA kit was found to be 32.5pg/ml. This was determined by adding 2 standard deviations to the mean OD obtained when the zero standard was assayed in 6 independent experiments. Intra-assay Precision: Reproducibility within the assay will be evaluated in three independent experiments. Each assay will be carried out with 6 replicates (3 duplicates) in 2 human pooled serum, 2 in RPMI and 2 in standard diluent with samples containing different concentrations of CD25. 2 standard curves were run on each plate The overall intra-assay coefficient of variation has been calculated to be 4.5%.
Inter-assay Precision: Assay to assay reproducibility within one laboratory will be evaluated in three independent experiments by two technicians. Each assay will be carried out with 6 replicates (3 duplicates) in 2 human pooled serum, 2 in RPMI and 2 in standard diluent with samples containing different concentrations of CD25. 2 standard curves were run on each plate. The calculated overall coefficient of variation was 10.3%.
Intended Uses: The sCD25 ELISA kit is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of sCD25 in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant human sCD25. Human CD25 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Brain Diseases, Charcot-Marie-Tooth Disease, Demyelinating Diseases, Disease Models, Animal, Hereditary Sensory and Motor Neuropathy, Muscle Weakness, Nerve Degeneration, Nervous System Diseases, Peripheral Nervous System Diseases, Tomaculous neuropathy are some of the diseases may be linked to Human CD25 ELISA Kit. Brain, Connective Tissue, Dorsal Root Ganglion, Embryonic Tissue, Eye, Heart, Kidney, Lung, Muscle, Ovary tissues are correlated with this protein.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions