CD141 elisa kit product blog
Tags: ELISA Kit; CD141; Human; No cross reactivity with other human soluble molecules ELISA Kit; CD141 / THROMBOMODULIN; CD141 elisa kit;
The Human. No cross reactivity with other human soluble molecules CD141 n/a (Catalog #MBS335473) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS335473 ELISA Kit recognizes Human. No cross reactivity with other human soluble molecules CD141.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
MyBioSource ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents, and a detailed procedure.
Sensitivity: The sensitivity or minimum detectable dose of CD141 using this CD141 ELISA kit was found to be 0.31 ng/ml. This was determined by adding 2 standard deviations to the mean OD obtained when the zero standard was assayed in 6 independent experiments. Intra-assay Precision: Reproducibility within the assay will be evaluated in three independent experiments. Each assay will be carried out with 6 replicates (3 duplicates) in 2 human pooled serum, 2 in RPMI and 2 in standard diluent with samples containing different concentrations of CD141. 2 standard curves were run on each plate The overall intra-assay coefficient of variation has been calculated to be 3.9%.
Inter-assay Precision: Assay to assay reproducibility within one laboratory will be evaluated in three independent experiments by two technicians. Each assay will be carried out with 6 replicates (3 duplicates) in 2 human pooled serum, 2 in RPMI and 2 in standard diluent with samples containing different concentrations of CD141. 2 standard curves were run on each plate. The calculated overall coefficient of variation was 9.8%. Human CD141 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.