CD138 elisa set product blog
Tags: CD138; ELISA Set; CD138 elisa set;
The CD138 n/a (Catalog #MBS335517) is an ELISA Set and is intended for research purposes only. The product is available for immediate purchase. The Murine CD138 ELISA Set reacts with Murine. No cross reactivity with other murine soluble molecule. and may cross-react with other species as described in the data sheet.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Intended Uses: The Mumps IgG Antibody ELISA Test Kit has been designed for the detection of IgG class antibodies against Mumps virus in serum and plasma.
Principle of the Assay: The Mumps IgG antibody test kit is based on the principle of the enzyme immunoassay (EIA). Mumps antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgG antibodies of the serum and the immobilized Mumps antigen takes place. After an one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgG peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgG antibodies is directly proportional to the intensity of the color.
Samples: Serum And Plasma. Intra-assay Precision: 8.9%
Inter-assay Precision: 7.8%.