CCL17 elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; CCL17/TARC; CCL17 elisa kit; CCL17;
The Human CCL17 ccl17 (Catalog #MBS175885) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS175885 ELISA Kit recognizes Human CCL17.The CCL17 ccl17 product has the following accession number(s) (GI #3024711) (NCBI Accession #Q92583.1) (Uniprot Accession #Q92583). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human CCL17/TARC PicoKine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only) (Human CCL17/TARC PicoKine ELISA Kit standard curve )
Principle of the assay: human CCL17 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL17 has been precoated onto 96-well plates. Standards (E.coli,A24-S94) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL17 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL17 amount of sample captured in plate.
Background: Chemokine (C-C motif) ligand 17 (CCL17) is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine (TARC). CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells.1 This chemokine specifically bindsand induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4.1,2The genefor CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1.3,4 Thestandard used in this kit is recombinant human CCL17, consisting of 71 amino acids with the molecular weight of 8Kda.
Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type: Sandwich
Detection Range: 31.2pg/ml-2000pg/ml
Sensitivity: < 1pg/ml. Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. Human CCL17/TARC PicoKine ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.