|The Casp1 casp1 (Catalog #MBS239178) is a Kit and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CASPASE-1 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS), Immunofluorescence (IF). Researchers should empirically determine the suitability of the Casp1 casp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Casp1 casp1 product has the following accession number(s) (GI #31542341) (NCBI Accession #NP_036894.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual
for complete product instructions).
Please refer to the product datasheet for known applications of a given kit. We\'ve tested the FAM FLICA CASPASE-1 KIT with the following immunoassay(s):
Testing Data #1 (Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases)
Description: The FAM FLICA Caspase 1 kit uses a quick and easy method to analyze active caspases in apoptotic cells.
Test Principle: Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on an unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe.
The Caspase FLICA Kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits. This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.
Reagents In The Kits: 1 vial of FAM-YVAD-FMK FLICA Reagent - lyophilized
10X Wash Buffer, 15 mL
Fixative, 6 mL
Propidium Iodide, 1 mL
Hoechst Stain, 1 mL. The following patways have been known to be associated with this gene. Adipose Tissue, Colon, Eye, Lung, Small Intestine, Spleen tissues are correlated with this protein. Casp1 also interacts with the following gene(s): Il18, Il1b, Il33, Naip2, Nfkb1, Nfkb2, Nlrp3, Pycard, Rela.