Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Blog  rightarrow C5 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • LACTB2 sirna blog
 • Eno1 recombinant protein blog
 • PYD elisa kit blog
 • NKD1 shrna blog
 • [Pyr6, Pro9]-Substance P peptide blog
 • anti-IgG antibody blog
 • chGH recombinant protein blog
 • ALDH6A1 shrna blog
 • Cit elisa kit blog
 • IQCF3 sirna blog
 • anti-ZADH1 antibody blog
 • PB19V elisa kit blog
 • TP53I11 shrna blog
 • HSPA6 blocking peptide blog
 • anti-17-BETA-ESTRADIOL antibody blog
 • CCL3 blocking peptide blog
 • anti-MCP1 antibody blog
 • C3a elisa kit blog
 • VCAM1 blocking peptide blog
 • IFI27L2 sirna blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • HSP-40 ELISA Kit
 • DAPP1 Antibody
 • Serum Amyloid A4 (SAA4) Recombinant Protein
 • CALR Antibody
 • Pulmonary surfactant-associated protein B (SFTPB) Recombinant Protein
 • ME3 Antibody
 • Myelin-oligodendrocyte glycoprotein (MOG) Recombinant Protein
 • anti-hepatitis B virus e antibody, HBeAb ELISA Kit
 • fibroblast growth factor 14 (FGF14) ELISA Kit
 • b-Amylase Antibody
 • Nuclear Receptor LXR alpha Antibody
 • ATP5A1 Antibody
 • GRB10 Antibody
 • Vitamin D3 (VD3) ELISA Kit
 downarrow more ...

C5 elisa kit product blog

Posted on 2014-12-09 15:56:46 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Rat ELISA Kit; C5 elisa kit; Complement Component 5;
The Rat C5 c5 (Catalog #MBS721993) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS721993 ELISA Kit recognizes Rat (Rattus, General) C5.

The C5 c5 product has the following accession number(s) (GI #109731812) (NCBI Accession #AAI13739.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat Complement Component 5 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) C5.

Principle of the assay: C5 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for C5. Standards or samples are then added to the microtiter plate wells and C5 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of C5 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for C5 are added to each well to "sandwich" the C5 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain C5 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C5 concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive or Sandwich
Detection Range: 25-500ng/mL
Sensitivity: 1.0ng/ml. Intended Uses: This C5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat C5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Rat Complement Component 5 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Bone, Bone Marrow, Brain, Eye, Intestine, Kidney, Liver, Lung, Muscle, Spleen tissues are correlated with this protein.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions