|The Mouse C1QL2 c1ql2 (Catalog #MBS909005) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS909005 ELISA Kit recognizes Mouse (Murine, General) C1QL2.
The C1QL2 c1ql2 product has the following accession number(s) (GI #46402221) (NCBI Accession #NP_997116.1) (Uniprot Accession #Q8CFR0). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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For SamplesFor the quantitative determination of mouse complement C1q-like protein 2 (C1QL2) concentrations in serum, plasma, tissue homogenates, cell lysates.
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C1QL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C1QL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C1QL2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C1QL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma, tissue homogenates, Cell lysates
Assay Type: Sandwich. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength: 450 nm
Sample Volume: 50-100ul. Mouse Complement C1q-like protein 2, C1QL2 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Brain tissues are correlated with this protein.