Brucella IgM elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; Brucella IgM; Brucella IgM elisa kit;
The Human Brucella IgM n/a (Catalog #MBS580091) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS580091 ELISA Kit recognizes Human Brucella IgM.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: The Brucella IgM Antibody ELISA Test Kit has been designed for the determination of specific IgM antibodies against Brucella in serum and plasma. For research use only.
Principle of the Assay: The Brucella IgM antibody test kit is based on the principle of the enzyme immunoassay (EIA). Brucella antigen is bound on the surface of the microtiter strips. Diluted unknowns are pipetted into the wells of the microtiter plate. A binding between the IgM antibodies of the serum and the immobilized Brucella antigen takes place. After an one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgM peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm.
Samples: Serum And Plasma. Brucella IgM ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.