|The Human BRCA2 brca2 (Catalog #MBS722169) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS722169 ELISA Kit recognizes Human (General) BRCA2.
The BRCA2 brca2 product has the following accession number(s) (GI #1161384) (NCBI Accession #AAB07223.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Breast Cancer Susceptibility Protein 2 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve
Intended Uses: This BRCA2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BRCA2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: BRCA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-BRCA2 antibody and an BRCA2-HRP conjugate. The assay sample and buffer are incubated together with BRCA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BRCA2 concentration since BRCA2 from samples and BRCA2-HRP conjugate compete for the anti-BRCA2 antibody binding site. Since the number of sites is limited, as more sites are occupied by BRCA2 from the sample, fewer sites are left to bind BRCA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BRCA2 concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 0.1 ng/mL. Human Breast Cancer Susceptibility Protein 2 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. BRCA2 also interacts with the following gene(s): ATM, BRCA1, FANCD2, FANCG, HMG20B, PALB2, RAD51, SHFM1, TP53, XRCC3.