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BRAF blocking peptide product blog

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Posted on 2017-04-20 21:27:14 by mybiosource_staff
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Tags: BRAF; B-raf; Blocking Peptide; BRAF blocking peptide;
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The BRAF braf (Catalog #MBS542476) is a Blocking Peptide and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s B-raf can be used in a range of immunoassay formats including, but not limited to, Immunodepletion, Immunocompetition.
ELISA: 1:10,000
Immunoprecipitation: 1:200
Western Blot: 1:500. Researchers should empirically determine the suitability of the BRAF braf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The BRAF braf product has the following accession number(s) (GI #33188459) (NCBI Accession #NP_004324.2) (Uniprot Accession #P15056). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Antigenic Blocking Peptide Serine/threonine-protein kinase B-raf Antibody
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

BRAF braf

Immunogen: Synthetic peptide taken within amino acid region 700-750 on human serine/threonine-protein kinase B-raf protein.
Expression: Brain and testis. Molecular Function: Kinase, Serine/threonine-protein kinase, Transferase
Structure: Monomer. Homodimer. Heterodimerizes with RAF1, and the heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer by phosphorylating BRAF at Thr-753. Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14. Interacts with RIT1. Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1. Interacts with DGKH. Interacts with PRMT5. Interacts with KSR2.
Subcellular Location: Nucleus, Cytoplasm, Cell membrane. Blood, Brain, Kidney, Lung, Lymph Node, Ovary, Pancreas, Prostate, Skin, Thyroid tissues are correlated with this protein. Carcinogenesis, Colorectal Neoplasms, Lung Diseases, Lung Neoplasms, Melanoma, Microsatellite Instability, Neoplasms, Experimental, Nervous System Diseases, Neuroendocrine Tumors, Thyroid Neoplasms are some of the diseases may be linked to B-raf Peptide. BRAF also interacts with the following gene(s): HRAS, KSR1, MAP2K1, MAP2K2, MAPK3, NRAS, PRKCA, RAF1, RAP1A.
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