Bme1390I restriction enzyme product blog
Tags: Restriction Enzyme; Bme1390I; Bme1390I restriction enzyme;
The Bme1390I n/a (Catalog #MBS638440) is a Restriction Enzyme produced from Bacillus megaterium RFL1390 and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given restriction enzyme. We\'ve tested the Bme1390I (ScrFI) with the following immunoassay(s):
Testing Data ()
5\'-C C^N G G-3\'.
Dilution Buffer: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol, or Storage Buffer.
Compatible Ends: CC^(C/G)GG-BcnI, SatI CC^(A/T)GG-MvaI, SatI
Methylation Effects: Bme1390I does not cut Cm5CNGG. Blocked by overlapping Dcm or CG methylation.
pACYC177: 14
pACYC184: 22. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Lambda: 184
Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Bme1390I, more than 80% of the DNA fragments can be ligated in a reaction mixture containing 20-40u of T4 DNA ligase/1ug of fragments and 10% PEG at a 5\'-termini concentration of 1.8uM. More than 90% of these can be recut.
M13mp18/19: 11
Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 320-fold overdigestion (20u/ug DNA x 16 hours) with Bme1390I.
pBR322: 16
PhiX174: 3
pTZ19R/U: 10
pUC18/19: 12
pUC57: 12
Stability During Prolonged Incubation: A minimum of 0.2units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25 degree C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation: Enzyme is inactivated by incubation at 80 degree C for 20min.
Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA dcm-in 1 hour at 37 degree C in 50ul of assay buffer.