|The BLOCK ACE n/a (Catalog #MBS238969) is an Accessory Reagent and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s BLOCK ACE can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB).
ELISA: Minimum Dilution: 1/4;
Western Blotting: Minimum Dilution: Neat. Researchers should empirically determine the suitability of the BLOCK ACE n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Block Ace is designed as a high-performance blocking reagent for use in immunological assays such as ELISA and Western blotting. It may also be used for dilution of antibodies and for washing procedures in the above assays. Block Ace has been shown to have superior performance to 1% BSA in blocking in ELISA assays. In comparison to BSA, Block Ace provides reduced backgrounds and sharper standard curves. For blocking in ELISA applications, we recommend using a 1/4 dilution of the reconstituted solution. For blocking in Western Blotting applications, we recommend using the reconstituted solution neat. For washing applications we recommend a 1/10 dilution of the reconstituted solution, and adding Tween 20 to a level of 0.05-0.2% v/v. For use as a test sample or secondary antibody diluent we recommend a 1/10 dilution of the reconstituted solution.
Reconstitution: Reconstitute each 4g sachet in 100ml distilled water.