bGH recombinant protein product blog
Tags: Recombinant Protein; BGrowth Hormone; bGH recombinant protein; bGH;
The bGH gh1 (Catalog #MBS143497) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase.The bGH gh1 product has the following accession number(s) (GI #30794288) (NCBI Accession #NP_851339.1) (Uniprot Accession #P01246). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including BGrowth Hormone are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.
Protein Content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.63 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of GH as a Reference Standard. Solubility: It is recommended to reconstitute the lyophilized Bovine Growth Hormone in 0.4% NaHCO3 or sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.68 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GH as a Reference Standard.
Biological Activity: The activity as determined by the PDFP13B9 cells stably transfected with rabbit GH receptors. Bovine GH is also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD).
Amino Acid Sequence: 1 - afpamslsgl fanavlraqh lhqlaadtfk efertyipeg qrysiqntqv
51 - afcfsetmpa ptgkneaqqk sdlellrisl lliqswlgpl qflsrvftns
101 - lvfgtsdrvy eklkdleegi lalmreledg tprrgqilkq tydkfdtnmr
151 - sddallknyg llscfrkdlh ktetylrvmk crrfgeasca f. Brain, Pituitary Gland tissues are correlated with this protein. The following patways have been known to be associated with this gene. Breast Neoplasms, Cardiovascular Diseases, Growth Disorders, Insulin Resistance, Isolated Growth Hormone Deficiency, Type II, Liver Diseases, Nervous System Diseases, Obesity, Pituitary Neoplasms, Weight Gain are some of the diseases may be linked to Recombinant Bovine Growth Hormone.