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beta-OHB elisa kit product blog

Posted on 2013-08-07 17:30:49 by mybiosource_staff
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Tags: ELISA Kit; Bovine ELISA Kit; beta-OHB elisa kit; beta-OHB; Beta-Hydroxybutyric Acid, beta-OHB;
The Bovine beta-OHB n/a (Catalog #MBS705232) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS705232 ELISA Kit recognizes Bovine (Cow, Cattle, General) beta-OHB.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Bovine Beta-Hydroxybutyric Acid, beta-OHB ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) beta-OHB.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with a goat-anti-rabbit antibody. Standards or samples are then added to the appropriate microtiter plate wells with a HRP-conjugated beta-OHB and antibody preparation specific for beta-OHB and incubated. Then substrate solutions are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of beta-OHB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Samples: Serum, plasma
Detection Range: 5 nmol/ml-80 nmol/ml
Sensitivity: 0.95 nmol/ml. Detection Wavelength: 450 nm
Sample Volume: 50-100ul. Bovine Beta-Hydroxybutyric Acid, beta-OHB ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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