Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Blog  rightarrow beta-EP elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • A1BG blocking peptide blog
 • Polr2a recombinant protein blog
 • NEUROD1 shrna blog
 • IgG immunoglobulin blog
 • ZNF572 shrna blog
 • anti-DDX58 antibody blog
 • anti-TBX1 antibody blog
 • anti-SERPINB13 antibody blog
 • SFRS12IP1 elisa kit blog
 • PNMA6A sirna blog
 • Hsp-70 elisa kit blog
 • CCR10 recombinant protein blog
 • SLC11A1 elisa kit blog
 • anti-MADD antibody blog
 • KRT83 elisa kit blog
 • Rubella Virus E1 Mosaic, Recombinant recombinant protein blog
 • Phytolacca americana lectin blog
 • TentaGel R RAM Resin (90 um), Rink-type resin blog
 • CAMKK2 shrna blog
 • USP17L20 sirna blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • Myokinase, yeast, MK Enzyme
 • IDH2 Antibody
 • Phospho Stathmin 1 (PSTMN1) ELISA Kit
 • SNAP25 (Ser187) Antibody
 • Chaperone SurA Antibody
 • Iron Responsive Element Binding Protein 2 (IREB2) ELISA Kit
 • CYTOKERATIN 7-17 (KRT17) Antibody
 • Choline acetyltransferase (ChAT) ELISA Kit
 • GFAP Peptide
 • GFAP Protein
 • p47 phox (p47-phox) Antibody
 • Orexins ELISA Kit
 • N (2)-fixation sustaining protein CowN (Hhal_0544) Recombinant Protein
 • Cluster of Differentiation 8 (CD8) ELISA Kit
 downarrow more ...

beta-EP elisa kit product blog

Posted on 2014-03-30 22:32:34 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Rabbit ELISA Kit; beta-EP elisa kit; beta-EP; Beta-Endorphin, beta-EP;
The Rabbit beta-EP n/a (Catalog #MBS720887) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS720887 ELISA Kit recognizes Rabbit (General) beta-EP.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rabbit Beta-Endorphin, beta-EP ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) beta-EP.

INTENDED USE: This BEP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit BEP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

PRINCIPLE OF THE ASSAY: BEP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BEP antibody and an BEP-HRP conjugate. The assay sample and buffer are incubated together with BEP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BEP concentration since BEP from samples and BEP-HRP conjugate compete for the anti-BEP antibody binding site. Since the number of sites is limited, as more sites are occupied by BEP from the sample, fewer sites are left to bind BEP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BEP concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0 pg/mL. Rabbit Beta-Endorphin, beta-EP ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions