BDNF recombinant protein product blog
Tags: BDNF; Recombinant Protein; Brain-Derived Neurotrophic Factor; BDNF recombinant protein;
The BDNF bdnf (Catalog #MBS142084) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase.The BDNF bdnf product has the following accession number(s) (GI #219842288) (NCBI Accession #NP_001137277.1) (Uniprot Accession #P23560). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including Brain-Derived Neurotrophic Factor are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.
Solubility: It is recommended to reconstitute the lyophilized BDNF in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content: BDNF quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.6 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of Brain-derived Neurotrophic Factor as a Reference Standard.
Biological Activity: The ED50, calculated by the dose-dependent induction of ACHE (acetylcholine esterase) in rat basal forebrain primary septal culture is 50 ng/ml corresponding to a Specific Activity of 20,000IU/mg. The following patways have been known to be associated with this gene. Body Weight, Brain Injuries, Brain Ischemia, Cognition Disorders, Depressive Disorder, Inflammation, Memory Disorders, Nerve Degeneration, Nervous System Diseases, Schizophrenia are some of the diseases may be linked to Recombinant Human Brain-Derived Neurotrophic Factor. Adrenal Gland, Brain, Connective Tissue, Embryonic Tissue, Eye, Heart, Liver, Lung, Skin, Thyroid tissues are correlated with this protein.