|The Human bCTx n/a (Catalog #MBS902219) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS902219 ELISA Kit recognizes Human (General) bCTx.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual
for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human beta-crosslaps, bCTx ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to bCTx. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated bCTx. A competitive inhibition reaction is launched between bCTx (Standards or samples) and Biotin-conjugated bCTx with the pre-coated antibody specific for bCTx. The more amount of bCTx in samples, the less antibody bound by Biotin-conjugated bCTx. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of bCTx in the sample. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma
Assay Type: Quantitative Competitive
Detection Range: 125 pg/ml - 2000 pg/ml
Sensitivity: Typically less than 125 pg/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess. Human beta-crosslaps, bCTx ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.