Barbiturates elisa kit product blog
Tags: ELISA Kit; Barbiturates; Barbiturates elisa kit;
The Barbiturates n/a (Catalog #MBS580058) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: The BAFF, soluble (human) ELISA kit (hypersensitive) is to be used for the measurement of human BAFF in serum, plasma and cell culture supernatant. This ELISA Kit is for research use only.
Principle of the Assay: This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for the measurement of human BAFF (BAFF) in serum, plasma and cell culture supernatant. A monoclonal antibody specific for BAFF has been pre-coated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, BAFF is recognized by the addition of a biotinylated monoclonal antibody specific for BAFF (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3\',5,5\'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of BAFF in the samples
Background/Introduction: BAFF (B cell activation factor of the TNF family, also known as BLyS or TALL1) is a key survival factor for peripheral B cells. BAFF is a homotrimeric type II transmembrane protein that can be proteolytically processed by furin to be released as soluble cytokine (1). Soluble BAFF adopts the classical trimeric form of the TNF-family ligand. However, BAFF has the unique property among the TNF-ligand to assemble as a 60-mer (2). BAFF is mainly produced by innate immune cells such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells. T cells, activated B cells, some malignant B cells and also non-lymphoid cells like astrocytes, synoviocytes and epithelial cells can also produce BAFF. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and together with IL-6, promotes Ig class-switching and plasma cell differentiation (1). Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of BAFF leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of persons with various autoimmune diseases (3), such as Sj�gren\'s syndrome (4), Rheumatoid Arthritis (RA) (5), Multiple sclerosis (MS) (6) and Systemic Lupus Erythematosus (SLE) (7). BAFF is also increased levels in some lymphoid cancers (8).
Samples: Serum, Plasma And Cell Culture Supernatant
Assay Type: Sandwich
Detection Range: 15.6 pg/ml-500 pg/ml
Sensitivity: 8 pg/ml. Intra-assay Precision: Five samples of known concentrations of human BAFF were assayed in replicates 16 times to test precision within an assay
Inter-assay Precision: Four samples of known concentrations of human BAFF were assayed in 4 separate assays to test precision between assays. Barbiturates ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.