B2M elisa kit product blog
Tags: ELISA Kit; B2M; beta-2 Microglobulin; B2M elisa kit;
The Rat B2M b2m (Catalog #MBS564134) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS564134 ELISA Kit recognizes Rat B2M.The B2M b2m product has the following accession number(s) (GI #179318) (Uniprot Accession #P61769). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Beta 2-Microglobulin present in samples reacts with the anti-Beta 2-Microglobulin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-B2M antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound B2M. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3\',5,5\'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of B2M in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of B2M in the test sample. The quantity of B2M in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Background: Beta 2-Microglobulin (B2M) is an 11 kDA protein. It forms the subunit of the MHC class I molecule and associates with the outer membrane of many cells including lymphocytes. It is present in low levels in serum and urine of normal people, but at a higher concentration in patients with renal diseases, kidney transplants and various other inflammatory and infectious conditions.
Samples: Immunoperoxidase Assay for Determination of Beta 2-Microglobulin in Rat Samples
Assay Type: Sandwich. Intended Uses: The Beta 2-Microglobulin test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Beta 2-Microglobulin in biological fluids of Rat. Rat beta 2-Microglobulin ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Blood, Bone, Bone Marrow, Brain, Heart, Kidney, Liver, Muscle, Skin, Spleen tissues are correlated with this protein. Cardiovascular Diseases, Female Urogenital Diseases, HIV Infections, Inflammation, Kidney Diseases, Leukemia, Lymphoma, Male Urogenital Diseases, Neoplasms, Renal Insufficiency are some of the diseases may be linked to Rat beta 2-Microglobulin ELISA Kit.