AT1R-AA elisa kit product blog
Tags: ELISA Kit; Rat ELISA Kit; Angiotensin 2 Receptor 1 Autoantibodies; AT1R-AA elisa kit; AT1R-AA;
The Rat AT1R-AA n/a (Catalog #MBS731165) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS731165 ELISA Kit recognizes Rat AT1R-AA.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat Angiotensin 2 Receptor 1 Autoantibodies ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This AT1R-AA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat AT1R-AA. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY AT1R-AA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with Angiotensin 2 Receptor 1 antigen. Standards or samples are then added to the microtiter plate wells and AT1R-AA if present, will bind to the antigen pre-coated wells. In order to quantitatively determine the amount of AT1R-AA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated Angiotensin 2 Receptor 1 antigen is added to each well to �sandwich� the AT1R-AA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AT1R-AA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AT1R-AA concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 0.5-10 ng/mL
Sensitivity: 0.1 ng/mL. Rat Angiotensin 2 Receptor 1 Autoantibodies ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.