|The Mouse AsAb n/a (Catalog #MBS282114) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS282114 ELISA Kit recognizes Mouse (Murine, General) AsAb.
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Principle of the Assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AsAb. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated AsAb and incubated. The competitive inhibition reaction is launched between with HRP labeled AsAb and unlabeled AsAb with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of AsAb in the sample. The color development is stopped and the intensity of the color is measured.
Samples: Serum, Plasma, Other biological fluids
Assay Type: Competitive ELISA. Sample Volume: 1-200 uL
Precision: Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Detection Wavelength: 450 nm. Mouse anti-sperm antibody (AsAb) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.