|The Mouse AsAb n/a (Catalog #MBS282114) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS282114 ELISA Kit recognizes Mouse (Murine, General) AsAb.
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(Or you can also download the PDF Manual
for complete product instructions).
Principle of the assay: The microtiter plate provided in this kit has been pre-coated with specific sperm antigen. Samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated AsAb and incubated. Then substrate solutions are added to each well. Only those wells that contain AsAb and enzyme-conjugated AsAb will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm.
Mouse anti-sperm antibody (AsAb) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.