anti-ZNF219 antibody product blog
Tags: Antibody; Polyclonal Antibody; ZFP219; anti-ZNF219 antibody; ZNF219;
The ZNF219 znf219 (Catalog #MBS150420) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZFP219 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s ZFP219 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).ZFP219 antibody can be used for detection of ZFP219 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the ZNF219 znf219 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ZNF219 znf219 product has the following accession number(s) (GI #156415996) (NCBI Accession #NP_001095142) (Uniprot Accession #Q9P2Y4). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ZFP219 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of ZFP219 in EL4 cell lysate with ZFP219 antibody at 1 μg/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of ZFP219 in mouse brain tissue with ZFP219 antibody at 5 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of ZFP219 in mouse brain tissue with ZFP219 antibody at 20 μg/mL.)
ZFP219 Antibody: ZFP219 is a developmentally regulated member of the Kruppel-like zinc finger gene family that is thought to function as a transcriptional repressor. Yeast two-hybrid screening showed association with Sox9, a transcription factor that is essential for chondrogenesis. ZFP219 is specifically expressed in the developing limb buds and colocalizes with Sox9 in the nucleus. Knockdown of ZFP219 expression decreased Sox9-induced mRNA expression, and a dominant-negative mutant of ZFP219 inhibited Bmp2-induced chondrocyte differentiation, suggesting that ZFP219 plays an important role as a transcriptional partner of Sox9 in the regulation of chondrocyte differentiation.
Conjugate: Unconjugated
Immunogen: ZFP219 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ZFP219.
Buffer: ZFP219 Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ZNF219 are readily searchable from our website. Different antibodies against the same target such as ZNF219 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.