anti-YPEL5 antibody product blog
Tags: Antibody; Polyclonal Antibody; YPEL5; anti-YPEL5 antibody;
The YPEL5 ypel5 (Catalog #MBS150769) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The YPEL5 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s YPEL5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF).YPEL5 antibody can be used for detection of YPEL5 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 2.5 mug/mL. For immunofluorescence start at 2.5 mug/mL. Researchers should empirically determine the suitability of the YPEL5 ypel5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The YPEL5 ypel5 product has the following accession number(s) (GI #7706341) (NCBI Accession #NP_057145) (Uniprot Accession #P62699). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the YPEL5 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of YPEL5 in A-20 cell lysate with YPEL5 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Immunocytochemistry (IHC) (Immunocytochemistry of YPEL5 in A20 cells with YPEL5 antibody at 2.5 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of YPEL5 in A20 cells with YPEL5 antibody at 5 μg/mL.)
YPEL5 Antibody: YPEL5 (yippee-like 5) belongs to a family of five yippee-like proteins, all of which localize to the centrosome or mitotic spindle and are widely expressed in both adult and fetal tissue. This localization plus the fact that the family of human YPEL proteins share a high degree of sequence homology across species suggests that these proteins may have a conserved function involved in cell division. YPEL5 is expressed at the nucleus and centrosome during interphase; during mitosis, it localizes to the spindle poles, mitotic spindle, and spindle midzone during mitosis. Finally, during cytokinesis, YPEL5 is localized to the midbody. It is associated with the Ran Binding Protein in the Microtubule organizing center (RanBPM) and a related protein RanBP10.
Conjugate: Unconjugated
Immunogen: YPEL5 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human YPEL5.
Buffer: YPEL5 Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing YPEL5 are readily searchable from our website. Different antibodies against the same target such as YPEL5 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.