anti-XAF1 antibody product blog
Tags: Antibody; Polyclonal Antibody; XAF-1; anti-XAF1 antibody; XAF1;
The XAF1 xaf1 (Catalog #MBS150176) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The XAF-1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s XAF-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).XAF-1 antibody can be used for the detection of XAF-1 by Western blot at 0.5 to 2 mug/mL. A 32 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 2 mug/mL. For immunofluorescence start at 10 mug/mL. Researchers should empirically determine the suitability of the XAF1 xaf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The XAF1 xaf1 product has the following accession number(s) (GI #1869901) (NCBI Accession #CAA68030) (Uniprot Accession #Q6GPH4). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the XAF-1 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of XAF-1 in human spleen lysate with XAF-1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL, respectively.)
Immunohistochemistry (IHC) (Immunohistochemical staining of human spleen tissue using XAF-1 antibody at 2 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of XAF-1 in Human Spleen tissue with XAF-1 antibody at 10 μg/mL.)
XAF-1 Antibody: XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP\'s ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines. Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.
Conjugate: Unconjugated
Immunogen: XAF-1 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human XAF-1.
Buffer: XAF-1 Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing XAF1 are readily searchable from our website. Different antibodies against the same target such as XAF1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.