|The WNT3A wnt3a (Catalog #MBS610874) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Wnt3a (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Wnt3a can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.5-2ug/ml detects hWnt-3a by indirect ELISA using 100ul/well antibody solution. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4ng/well of recombinant hWnt-3a.
Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWnt-3a is 1.5-3ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the WNT3A wnt3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The WNT3A wnt3a product has the following accession number(s) (GI #14530679) (Uniprot Accession #P56704). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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The Wnt gene family consists of structurally related genes, which encode secreted signaling proteins.1 Wnt proteins have been implicated in oncogenesis 2-3 and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the Wnt-beta-catenin-TCF signaling pathway.3.
Immunogen: Recombinant Wnt3a. Positive Control: Human Wnt3 transfected 293 cells. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing WNT3A are readily searchable from our website. Different antibodies against the same target such as WNT3A may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Connective Tissue, Lung, Placenta, Testis, Uncharacterized Tissue tissues are correlated with this protein.