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anti-WNT10B antibody product blog

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Posted on 2014-03-15 21:38:50 by mybiosource_staff
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Tags: Antibody; WNT10B; Monoclonal Antibody; anti-WNT10B antibody;
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The WNT10B wnt10b (Catalog #MBS831310) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The WNT10B antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s WNT10B can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB).
ELISA: 1:10,000
IF: 1:200-1:1000
IHC: 1:200-1:1000
WB: 1:500-1:2000. Researchers should empirically determine the suitability of the WNT10B wnt10b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The WNT10B wnt10b product has the following accession number(s) (GI #1932789) (NCBI Accession #AAB51685.1) (Uniprot Accession #O00744). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the WNT10B antibody with the following immunoassay(s):
Western Blot (WB) (Western Blot showing WNT10B antibody used against Hela cell lysate (1).)
Western Blot (WB) WNT10B.

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human normal stomach (A), normal liver (B), normal kidney (C) and rectum cancer tissues (D) using WNT10B antibody with DAB staining.)
Immunohistochemistry (IHC) WNT10B.

Mouse monoclonal WNT10B antibody.

Biological Significance: WNT10B is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10B protein at the amino acid level. The WNT10B gene is clustered with another family member, WNT1, in the chromosome 12q13 region. Immunogen: WNT10B antibody was raised in Mouse using a purified recombinant fragment of human WNT10B expressed in E Coli as the immunogen. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing WNT10B are readily searchable from our website. Different antibodies against the same target such as WNT10B may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. WNT10B also interacts with the following gene(s): FABP4, FZD1, FZD2, FZD3, FZD4, FZD7, LRP6, PPARG, SFRP1, SFRP2. The following patways have been known to be associated with this gene. Breast Neoplasms, Carcinoma, Hepatocellular, Congenital Abnormalities, Fibrosis, Hyperplasia, Inflammation, Liver Diseases, Necrosis, Neoplasms, Experimental, Weight Gain are some of the diseases may be linked to WNT10B antibody.
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