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anti-WNT-1 antibody product blog

Posted on 2014-08-28 23:32:46 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; WNT-1; anti-WNT-1 antibody;
The WNT-1 wnt1 (Catalog #MBS222769) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s WNT-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB).
ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml;
Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml. Researchers should empirically determine the suitability of the WNT-1 wnt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The WNT-1 wnt1 product has the following accession number(s) (GI #4885655) (NCBI Accession #NP_005421.1) (Uniprot Accession #P04628). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RABBIT ANTI HUMAN WNT-1 with the following immunoassay(s):
Testing Data #1 (Formalin fixed, paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human WNT-1 (MBS222769) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Testing Data #1 WNT-1.

Testing Data #2 (Formalin fixed, paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human WNT-1 (MBS222769) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Testing Data #2 WNT-1.

Rabbit anti Human WNT-1 antibody specifically recognizes Wnt-1, formerly known as int-1, a 343 amino acid non-glycosylated secreted protein associated with the extracellular matrix, involved in normal embryonic development and particularly linked with breast cancer. Wnt-1, which signals through binding to the Frizzled family of transmembrane receptors, was originally identified as a proto-oncogene implicated in carcinomas induced by the mouse mammary tumour virus (MMTV). Wnt-1 belongs to a family of Wnt genes involved in the dorso-ventricular development of the mesoderm during embryogenesis, with a restricted temporal and spatial pattern of expression.

Reconstitution: Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. Immunogen: Recombinant human Wnt-1
Buffer Solution: Phosphate buffered saline
Antiserum Preparation: Antisera to human Wnt-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing WNT-1 are readily searchable from our website. Different antibodies against the same target such as WNT-1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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