anti-Wisp2 antibody product blog
Tags: Antibody; WISP2; Monoclonal Antibody; anti-WISP2 antibody; CTGFL;
The Wisp2 wisp2 (Catalog #MBS605952) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CTGFL (Connective Tissue Growth Factor-Like Protein, WISP-2, Wnt1-Inducible-Signaling Pathway Protein 2) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CTGFL can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.05-0.2ug/ml
Western Blot: 0.1-1ug/ml. Researchers should empirically determine the suitability of the Wisp2 wisp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Wisp2 wisp2 product has the following accession number(s) (GI #4337060) (Uniprot Accession #Q9Z0G4). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CTGFL (Connective Tissue Growth Factor-Like Protein, WISP-2, Wnt1-Inducible-Signaling Pathway Protein 2) with the following immunoassay(s):
Testing Data (Lane 1, 2, 3 and 6: CTGFL full length recombinant protein with GST tag in 293 cell lysate|Lane 4: 10ug 293 cell lysate|Lane 5: GST protein|A: MBS605952 (1ug/ml). |B: Negative control|Secondary antibody: IgG (HRP) Goat Anti-Mouse)
CTGFL is a member of the connective tissue growth factor/cysteine-rich 61/nephroblastoma overexpressed (nov) (CCN) family and is upregulated in the mouse mammary epithelial cell line C57MG transformed by Wnt-1 and in several non-invasive human breast tumor cell lines. CTGFL expression is enhanced by important modulators of human breast cancer cell proliferation such as estrogen, progesterone and epidermal growth factor (EGF) in MCF-7 cells. These effects, inhibited by appropriate antagonists, indicate that steroids and growth factor-induced upregulation of CTGFL may be mediated through receptors. CTGFL gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two CTGFL members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover.
Immunogen: Full length recombinant CTGFL with GST tag. Hybridoma: Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Wisp2 are readily searchable from our website. Different antibodies against the same target such as Wisp2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.