anti-WIF1 antibody product blog
Tags: Antibody; WIF1; Polyclonal Antibody; anti-WIF1 antibody;
The WIF1 wif1 (Catalog #MBS627144) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WIF1, CT (Wnt Inhibitory Factor 1, WIF-1, UNQ191/PRO217) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s WIF1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the WIF1 wif1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The WIF1 wif1 product has the following accession number(s) (GI #111125011) (NCBI Accession #NP_009122.2) (Uniprot Accession #Q9Y5W5). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the WIF1, CT (Wnt Inhibitory Factor 1, WIF-1, UNQ191/PRO217) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of WIF1 (arrow) using rabbit polyclonal WIF1 (C-term) Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the WIF1 gene (Lane 2) (Origene Technologies).)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with WIF1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WNT proteins are extracellular signaling molecules involved in the control of embryonic development. WIF1 is a secreted protein, which binds WNT proteins and inhibits their activities. This protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals.
Immunogen: Synthetic peptide selected from the C-term region of human WIF1 (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing WIF1 are readily searchable from our website. Different antibodies against the same target such as WIF1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.