anti-VTCN1 antibody product blog
Tags: Antibody; Monoclonal Antibody; VTCN1 / B7-H4; anti-VTCN1 antibody; VTCN1;
The VTCN1 vtcn1 (Catalog #MBS246437) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone H74] (IgG1,k) to Human VTCN1 / B7-H4 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s VTCN1 / B7-H4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Flow Cytometry (FC/FACS).IHC-Paraffin (20ug/ml)
(optimal dilution to be determined by the researcher)
This H74 antibody has been tested by flow cytometric analysis of transient hB7-H4 transfected cells. This can be used at less than or equal to 0.25 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the VTCN1 vtcn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The VTCN1 vtcn1 product has the following accession number(s) (GI #99028881) (NCBI Accession #NP_078902.2) (Uniprot Accession #Q7Z7D3). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Monoclonal [clone H74] (IgG1,k) to Human VTCN1 / B7-H4 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-VTCN1 / B7-H4 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)
Immunohistochemistry (IHC) (Anti-VTCN1 / B7-H4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)
Flow Cytometry (FC/FACS) (Staining of human B7-H4 transfected 293T cells with 0.125 ug of Purified Mouse IgG1, K isotype control (open histogram) or 0.125 ug of Purified anti-human B7-H4 (H74) (colored histogram) followed by Biotin Anti-Mouse IgG and SAv-PE. Total cells were used for analysis.)
Flow Cytometry (FC/FACS) (Staining of non-transfected 293T cells with 0.125 ug of Purified Mouse IgG1, K isotype control (open histogram) or 0.125 ug of Purified anti-human B7-H4 (H74) (colored histogram) followed by Biotin Anti-Mouse IgG and SAv-PE. Total cells were used for analysis.)
Target Species: Human
Immunogen Description: VTCN1/B7-H4 antibody was raised against human VTCN1. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:
Tissue Preparation:
Formalin fixation and embedding in paraffin wax.
Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60�C.
Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT
Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT
Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100�C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT
Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply an universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT
Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip .