anti-VEGFR1 antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-VEGFR1 antibody; VEGFR1;
The VEGFR1 flt1 (Catalog #MBS832207) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s VEGFR1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).FC: <= 1 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest. Researchers should empirically determine the suitability of the VEGFR1 flt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The VEGFR1 flt1 product has the following accession number(s) (GI #229892302) (NCBI Accession #NP_001153503.1) (Uniprot Accession #P17948). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the VEGFR1 antibody with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of non-boiled NP-40 lysates from HUVEC cells using with VEGFR1 antibody (2 ug/ml) followed by Rat IgG HRP antibody.)
Rat monoclonal VEGFR1 antibody.
Biological Significance: This 7A6 monoclonal antibody recognizes human VEGFR1. It is directed against the extracellular domain and can recognize both membrane-bound and soluble forms of VEGFR1, with approximate molecular weights of 180 kDa and 90 kDa, respectively. Mice lacking expression of VEGFR1 have disorganized vasculature and die in utero, however angiogenesis does not require the tyrosine kinase activity of VEGFR1 as expression of a membrane-bound form lacking the tyrosine kinase domain allows normal development. Although fertile and viable, mice lacking the tyrosine kinase domain of VEGFR1 do have a defect in the migration of macrophages. The soluble form of VEGFR1 can be detected in the serum of normal patients, and elevated levels of soluble VEGFR1 in pregnant women is associated with preeclampsia. Several tumor cell lines have been reported to express membrane-bound VEGFR1, including malignancies of hematopoietic origin. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing VEGFR1 are readily searchable from our website. Different antibodies against the same target such as VEGFR1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Brain Diseases, Disease Models, Animal, Heart Diseases, Hypertension, Inflammation, Kidney Diseases, Necrosis, Neoplasms, Experimental, Pre-Eclampsia, Proteinuria are some of the diseases may be linked to VEGFR1 antibody. Blood, Bone, Embryonic Tissue, Heart, Kidney, Liver, Lung, Muscle, Placenta, Vascular tissues are correlated with this protein. VEGFR1 also interacts with the following gene(s): EPAS1, HIF1A, NRP1, NRP2, PGF, SHC1, VEGFA, VEGFB.