|The VCP vcp (Catalog #MBS245142) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 4G9] (IgG1) to Human VCP reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s VCP can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP).
IF, IHC-P (5 ug/ml), IP, WB
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the VCP vcp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The VCP vcp product has the following accession number(s) (GI #6005942) (NCBI Accession #NP_009057.1) (Uniprot Accession #P55072). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Monoclonal [clone 4G9] (IgG1) to Human VCP with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-VCP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunocytochemistry (ICC) (Overnight nocodazole treated Hela cells stained with purified mouse monoclonal antibody against p97/VCP (clone 4G9), followed by Cy3 conjugated goat anti-mouse IgG and DAPI)
Western Blot (WB) (Western blot of extracts from HeLa cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9)
Valsolin Containing Protein (VCP) is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex.
Target Species: Human
Immunogen Description: Recombinant human VCP. Immunogen Type: Recombinant protein
Immunogen: VCP antibody was raised against recombinant human VCP. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing VCP are readily searchable from our website. Different antibodies against the same target such as VCP may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.