anti-VAPB antibody product blog
Tags: Antibody; Monoclonal Antibody; VAPB; anti-VAPB antibody;
The VAPB vapb (Catalog #MBS6011828) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The VAPB (Vesicle-associated Membrane Protein-associated Protein B/C, VAMP-B/VAMP-C, VAMP-associated Protein B/C, VAP-B/VAP-C, UNQ484/PRO983) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s VAPB can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC).Suitable for use in Immunohistochemistry.
Dilution: Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human brain (cerebellum)
Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the VAPB vapb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The VAPB vapb product has the following accession number(s) (GI #37182052) (NCBI Accession #AAQ88829.1) (Uniprot Accession #O95292). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the VAPB (Vesicle-associated Membrane Protein-associated Protein B/C, VAMP-B/VAMP-C, VAMP-associated Protein B/C, VAP-B/VAP-C, UNQ484/PRO983) with the following immunoassay(s):
Immunohistochemistry (IHC) (VAP-B in Human Brain. VAP-B was detected in immersion fixed paraffin- embedded sections of human brain (cerebellum) using MBS6011828 at 15ug/ml overnight at 4°C. Tissue was stained using the Mouse HRP-DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies.)
Vesicle-associated membrane protein associated protein B is an~30kD ubiquitously expressed type IV transmembrane protein belonging to the VAP family. It is found in endoplasmic reticulum (ER), Golgi and other membranes as a homodimer or a heterodimer with VAP-A, probably associating through a GxxxG motif in the transmembrane regions. Human VAP-B cDNA encodes 243aa that include a 222aa cytoplasmic domain and a 21aa C-terminal membrane anchor. The cytoplasmic domain contains a mobile sperm protein (MSP) domain aa7-124 and a coiled-coil region aa159-196. Human VAP-B shares 90%, 89%, 96%, 96% and 94% aa identity with mouse, rat, canine, bovine and porcine VAP-B, respectively. VAP-An and VAP-B MSP domains recruit FFAT motif-containing proteins to the cytosolic surface of ER membranes. FFAT proteins mediate many of the effects of VAPs on regulation of membrane transport, phospholipid biosynthesis, microtubule organization, and the unfolded protein response. VAPs also interact with some SNARE and viral proteins. A human polymorphism of VAP-B, P56S, is found in three familial motor neuron diseases, notably the amylotrophic lateral sclerosis variant ALS8. It produces a non-functional protein that can dimerize with and inhibit function of normal VAP-B, leading formation of intracellular aggregates and increased ER-stress-induced death of motor neurons. It can also promote cleavage and secretion of soluble VAP-B, which can then function as a ligand for EPH receptors. A naturally occurring 99aa isoform of VAP-B that diverges at 71aa within the MSP domain is termed VAP-C. It also appears to be a negative regulator of VAP-An and VAP-B. While VAP-B is used by hepatitis C virus (HCV) for its propagation, VAP-C inhibits HCV propagation.