anti-UBAP1 antibody product blog
Tags: Antibody; Polyclonal Antibody; UBAP1; anti-UBAP1 antibody;
The UBAP1 ubap1 (Catalog #MBS629580) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBAP1, NT (Nasopharyngeal carcinoma-associated gene 20 protein, UAP, UBAP, UBAP-1, Ubiquitin-associated protein 1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s UBAP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500. Researchers should empirically determine the suitability of the UBAP1 ubap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The UBAP1 ubap1 product has the following accession number(s) (GI #283945560) (NCBI Accession #NP_001164673.1) (Uniprot Accession #Q9NZ09). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the UBAP1, NT (Nasopharyngeal carcinoma-associated gene 20 protein, UAP, UBAP, UBAP-1, Ubiquitin-associated protein 1) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of UBAP1 Antibody (N-term) in mouse liver, cerebellum tissue lysates (35ug/lane). UBAP1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain tissue reacted with UBAP1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (UBAP1 Antibody (N-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
UBAP1 is a member of the UBA domain family, whose members include proteins having connections to ubiquitin and the ubiquitination pathway. The ubiquitin associated domain is thought to be a non-covalent ubiquitin binding domain consisting of a compact three helix bundle. This particular protein riginates from a gene locus in a refined region on chromosome 9 undergoing loss of heterozygosity in nasopharyngeal carcinoma (NPC). Taking into account its cytogenetic location, this UBA domain family member is being studies as a putative target for mutation in nasopharyngeal carcinomas.
Immunogen: Synthetic peptide selected from the N-terminal region of human UBAP1 (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing UBAP1 are readily searchable from our website. Different antibodies against the same target such as UBAP1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.