anti-TP53 antibody product blog
Tags: Antibody; Polyclonal Antibody; TP53; anti-TP53 antibody; p53, acetylated;
The TP53 tp53 (Catalog #MBS617027) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p53, acetylated (Lys373) (Tumor Suppressor Protein, Oncogene Protein) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s p53, acetylated can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP).Suitable for use in Immunoblot and Immunoprecipitation.
Dilution: Immunoblot Analysis:1:1000-1:5000 detects GST-p53 acetylated by recombinant p300, HAT domain in an in vitro acetyl transferase assay. Researchers should empirically determine the suitability of the TP53 tp53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TP53 tp53 product has the following accession number(s) (GI #23491729) (Uniprot Accession #P04637). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the p53, acetylated (Lys373) (Tumor Suppressor Protein, Oncogene Protein) with the following immunoassay(s):
Chemiluminescence (Representative blot from a previous lot. In vitro acetyl transferase reaction products were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS617027 (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Lane 1: GST-p53 alone; lane 2: GSTp53 in the presence of PCAF; lane 3: GST-p53 in the presence of p300. Arrow indicates acetylated GST-p53.)
p53 tumor suppressor gene mutations are one of the most common genetic alterations in human tumors, including lymphomas. Altering or inactivating p53 by mutation or reaction with oncogenes or viruses may cause depletion of p53, or conversion of p53 from a tumor suppressor gene to a transforming oncogene. The nuclear protein p53 is associated with cell transformation and proliferation, with definitive possibilities as a marker in malignant lymphoma progression. p53\'s role in the multistep process leading to cancer is still being investigated, with an emphasis on its relation and control over additional tumor suppressors.
Immunogen: KLH-conjugated, synthetic peptide corresponding to aa367-379 of human p53 (SHLKSK[AcK]GQSTSR, where AcK denotes acetyllysine). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TP53 are readily searchable from our website. Different antibodies against the same target such as TP53 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.