|The TOP2A top2a (Catalog #MBS125825) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TOP2A Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s TOP2A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:20 - 1:50. Researchers should empirically determine the suitability of the TOP2A top2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TOP2A top2a product has the following accession number(s) (GI #13959709) (NCBI Accession #P11388.3) (Uniprot Accession #P11388). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the TOP2A Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using TOP2A antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.)
TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. The antibody is specific to the gene TOP2A.
Route: Synthetic Peptide. Immunogen: A synthetic peptide of human TOP2A
Calculated Molecular Weight: 174kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TOP2A are readily searchable from our website. Different antibodies against the same target such as TOP2A may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Adenocarcinoma, Breast Neoplasms, Carcinoma, Leukemia, Liver Neoplasms, Lung Neoplasms, Neoplasms, Experimental, Nervous System Diseases, Ovarian Neoplasms, Uterine Cervical Neoplasms are some of the diseases may be linked to TOP2A Polyclonal Antibody. Blood, Bone, Bone Marrow, Brain, Liver, Lung, Lymph, Lymph Node, Prostate, Stomach tissues are correlated with this protein.