anti-TNFRSF12A antibody product blog
Tags: Antibody; Polyclonal Antibody; Fn14; anti-TNFRSF12A antibody; TNFRSF12A;
The TNFRSF12A tnfrsf12a (Catalog #MBS150342) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Fn14 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Fn14 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).Fn14 antibody can be used for the detection of Fn14 by Western blot at 2 - 4 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the TNFRSF12A tnfrsf12a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TNFRSF12A tnfrsf12a product has the following accession number(s) (GI #7706186) (NCBI Accession #NP_057723) (Uniprot Accession #Q9NP84). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Fn14 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of Fn14 in HepG2 cells with Fn14 antibody at (A) 2 and (B) 4 μg/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of Fn14 in human liver tissue with Fn14 antibody at 2.5 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of Fn14 in Human Liver cells with Fn14 antibody at 20 μg/mL.)
Fn14 Antibody: TWEAK is a TNF family member produced by IFN-gamma-stimulated monocytes and induces multiple cell death pathways including caspase-dependent apoptosis and cathepsin B-dependent necrosis. These pathways are mediated by the binding of TWEAK by fibroblast growth factor 14 (Fn14), a member of the TNF receptor superfamily. Yeast two-hybrid experiments have shown that FN14 will bind to the TNFR-associated factors TRAF1, TRAF2, TRAF3, and TRAF5, suggesting that the signaling pathway induced by TWEAK may be mediated by one or more of these adaptor molecules. Fn14 is induced during liver regeneration and is highly expressed in hepatocellular carcinomas. In addition, TWEAK induces liver progenitor cell proliferation, suggesting Fn14 may play a role in hepatocyte growth control and liver neoplasia.
Conjugate: Unconjugated
Immunogen: Fn14 antibody was raised against a human IgG1 Fc-tagged Fn14 protein.
Buffer: Fn14 Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TNFRSF12A are readily searchable from our website. Different antibodies against the same target such as TNFRSF12A may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.