anti-TNF antibody product blog
Tags: Antibody; TNF; Monoclonal Antibody; anti-TNF antibody; TNF-alpha;
The TNF tnf (Catalog #MBS550019) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal mouse anti human TNF-alpha reacts with Human and may cross-react with other species as described in the data sheet.ELISA: This antibody can be used at 4 ug/mL with the appropriate secondary reagents to detect TNF alpha. The detection limit for recombinant human TNF alpha is approximately 0.1 ng/well. Researchers should empirically determine the suitability of the TNF tnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TNF tnf product has the following accession number(s) (GI #25952111) (NCBI Accession #NP_000585.2) (Uniprot Accession #P01375). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Tumor Necrosis Factor is secreted by macrophages, monocytes, neutrophils, T-cells, NK- cells following their stimulation by bacterial lipopolysaccharides. Cells expressing CD4 secrete TNF- alpha while CD8 (+) cells secrete little or no TNF- alpha. Stimulated peripheral neutrophilic granulocytes but also unstimulated cells and also a number of transformed cell lines, astrocytes, microglial cells, smooth muscle cells, and fibroblasts also secrete TNF. Human milk also contains this factor.
Host Note: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human TNFa. Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TNF are readily searchable from our website. Different antibodies against the same target such as TNF may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Brain, Connective Tissue, Heart, Intestine, Lung, Lymph Node, Ovary, Placenta, Prostate tissues are correlated with this protein. Arthritis, Rheumatoid, Cardiovascular Diseases, Infection, Inflammation, Leukemia, Liver Diseases, Lung Diseases, Neoplasms, Sepsis, Skin Diseases are some of the diseases may be linked to Monoclonal mouse anti human TNF-alpha. The following patways have been known to be associated with this gene. TNF also interacts with the following gene(s): ADAM17, IFNG, TNFRSF1A, TRAF2.