anti-TNF ALPHA antibody product blog
Tags: Antibody; Monoclonal Antibody; TNF alpha; anti-TNF ALPHA antibody;
The TNF ALPHA tnf (Catalog #MBS212214) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s TNF ALPHA can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS)*.Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
ELISA: Minimum Dilution: 5ug/ml; Maximum Dilution: 10ug/ml
Flow Cytometry: Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose. Researchers should empirically determine the suitability of the TNF ALPHA tnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TNF ALPHA tnf product has the following accession number(s) (GI #25952111) (NCBI Accession #NP_000585.2) (Uniprot Accession #P01375). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Rat anti Human TNF alpha antibody, clone MP9-20A4 recognizes human tumor Necrosis Factor - alpha (TNF alpha), also known as TNFSF2. This 17kDa cytokine is expressed by activated macrophages, monocytes, neutrophils, T cells and NK cells. Rat anti Human TNF alpha antibody, clone MP9-20A4 is reported to act as a blocking antibody (Abrams et al. 2001).
Perservative Stabilisers: 0.09% Sodium Azide
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Buffer Solution: Phosphate buffered saline
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TNF ALPHA are readily searchable from our website. Different antibodies against the same target such as TNF ALPHA may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. TNF ALPHA also interacts with the following gene(s): BIRC2, CD40, ICAM1, IL1B, IL6, RIPK1, TNFRSF1A, TNFRSF1B, TRADD, TRAF2. The following patways have been known to be associated with this gene. Atherosclerosis, Colitis, Fibrosis, Inflammation, Insulin Resistance, Liver Diseases, Lung Diseases, Necrosis, Pain, Vascular Diseases are some of the diseases may be linked to RAT ANTI HUMAN TNF ALPHA. Blood, Brain, Connective Tissue, Heart, Intestine, Lung, Lymph Node, Ovary, Placenta, Prostate tissues are correlated with this protein.