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anti-TLR9 antibody product blog

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Posted on 2013-11-10 10:57:26 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; TLR9; anti-TLR9 antibody;
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The TLR9 tlr9 (Catalog #MBS245240) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 26C593.2] (IgG1,k) to Human TLR9 reacts with Gibbon, Gorilla, Human, Mouse, Orangutan, Rat
Predicted Reactivity: Chimpanzee, Monkey (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. MyBioSource\'s TLR9 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB), Flow Cytometry (FC/FACS).
Flo, IHC-Fr (10 - 20 ug/ml), IHC-P (5 ug/ml), WB (1 - 3 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the TLR9 tlr9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The TLR9 tlr9 product has the following accession number(s) (GI #8394456) (NCBI Accession #NP_059138.1) (Uniprot Accession #Q9NR96). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Monoclonal [clone 26C593.2] (IgG1,k) to Human TLR9 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-TLR9 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC) TLR9.

Flow Cytometry (FC/FACS) (Intracellular flow analysis of TLR9 in Ramos cells using 0.1 ug of antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control; red represents anti-TLR9 antibody.)
Flow Cytometry (FC/FACS) TLR9.

Western Blot (WB) (Western blot of TLR9 in A) human PBMC, B) human intestine, C) mouse intestine, and D) rat intestine tissue lysates using antibody at a dilution of 3 ug/ml. Lane E shows antibody tested at 5 ug/ml in human MCF7 cell lysate.)
Western Blot (WB) TLR9.

Target Species: Human
Immunogen Description: This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-284 (CPRHFPQLHPDTFSHLS) of human TLR9 isoform A (Genbank accession no. AAF78037). Immunogen Type: Synthetic peptide - KLH conjugated
Immunogen: TLR9 antibody was raised against this antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-284 (CPRHFPQLHPDTFSHLS) of human TLR9 isoform A (Genbank accession no. AAF78037). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%); Chimpanzee, Marmoset (94%); Sheep, Goat, Zebu, Bovine, Horse (82%).
Antigen Modification: aa268-284. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TLR9 are readily searchable from our website. Different antibodies against the same target such as TLR9 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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