anti-Thrombin antibody product blog
Tags: Antibody; Thrombin; Polyclonal Antibody; anti-Thrombin antibody;
The Thrombin n/a (Catalog #MBS512149) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase.Suitable as a source of antibodies to human thrombin (and prothrombin). Researchers should empirically determine the suitability of the Thrombin n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Thrombin n/a product has the following accession number(s) (GI #213487). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Thrombin (EC3.4.21.5, alpha-thrombin) is the product of proteolytic activation of the zymogen prothrombin. Human thrombin is a two-chain serine protease with a mass of 37 kDa. The active site is located within the heavy chain. Thrombin has a high specificity for certain arginine bonds in protein substrates. The primary substrate is fibrinogen which thrombin converts to fibrin through the cleavage of four arginyl-glycyl peptide bonds. Thrombin is also an important activator of platelets, factor XIII, Protein C and TAFI (Plasma procarboxypeptidase B). In a positive feedback mechanism, thrombin increases the rate of its own production by activation of factors VIII and V. The rate of thrombin production is subsequently limited indirectly through the activation of Protein C by thrombin, which then inactivates the activated cofactors VIII and V. The binding of thrombin to thrombomodulin on the cell surface dramatically alters thrombin\'s specificity, increasing it\'s activity toward Protein C and TAFI, and decreasing it\'s activity toward fibrinogen and activating cofactors VIII and V. In plasma, thrombin activity is inhibited primarily by antithrombin and to a lesser extent heparin cofactor II. The rate of inhibition by both of these inhibitors is profoundly increased in the presence of optimal concentrations of heparin. Other physiological inhibitors of thrombin in the absence of heparin include alpha2-macroglobulin and alpha1-antitrypsin1-4.
Immunogen: Thrombin prepared from purified human prothrombin, active site blocked with PPACK. Buffer: 10 mM HEPES, pH 7.4, 150 mM NaCl, 50% (v/v) glycerol.
Neutralizing Activity: Not determined. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Thrombin are readily searchable from our website. Different antibodies against the same target such as Thrombin may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.