anti-TGFB1 antibody product blog
Tags: Antibody; Polyclonal Antibody; TGFB1; anti-TGFB1 antibody; TGF-beta 1;
The TGFB1 tgfb1 (Catalog #MBS551006) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti TGF-beta 1 Polyclonal reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s TGF-beta 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA).ELISA: Use at a concentration range of 1-2ug/ml.
Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml.
IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.
Immunohistochemical analysis of TGF beta 1 expression in rat articular cartilage tissue.
It was used as primary antibody dilution 1:500.
Immunohistochemical analysis of TGF beta 1 expression in human pancreatic tissue.
It was used as primary antibody, dilution 1:500. Researchers should empirically determine the suitability of the TGFB1 tgfb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TGFB1 tgfb1 product has the following accession number(s) (GI #63025222) (NCBI Accession #NP_000651.3) (Uniprot Accession #P01137). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti TGF-beta 1 Polyclonal with the following immunoassay(s):
Immunohistochemistry
TGF-beta is the prototype of a protein family known as the TGF-beta superfamily. This family includes Inhibins, Activin A, MIS (Müllerian inhibiting substance), BMP (bone morphogenetic proteins), dpp (decapentaplegic) and Vg-1. Type 1 receptors and type 2 receptors have a molecular mass of 53 and 75 kDa, respectively. Signaling through the receptor requires heterodimeric complexes between both types of receptors. The type 2 receptor has been shown to bind the ligand, but it is incapable of mediating TGF- beta responses in the absence of the type 1 receptor. The type 1 receptor is expressed predominantly in hematopoietic progenitor cells. The type 2 receptor encodes a protein with an intracellular domain that functions as a serine/threonine kinase. TGF-beta is the most potent known growth inhibitor for normal and transformed epithelial cells, endothelial cells, fibroblasts, neuronal cells, lymphoid cells and other hematopoietic cell types. TGF-beta inhibits the proliferation of T- lymphocytes by down- regulating predominantly IL- 2 mediated proliferative signals. It also inhibits the growth of natural killer cells in vivo and deactivates macrophages. TGF-beta blocks the antitumor activity mediated in vivo by IL-2 and transferred lymphokine- activated or tumor infiltrating lymphocytes.
Source Note: Polyclonal TGF-beta 1 antibody was produced from sera of rabbits immunized with synthetic protein containing TGF-beta 1 antigenic determinants. Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers.