anti-TCR, alpha, beta, V beta 1 antibody product blog
Tags: Antibody; Monoclonal Antibody; TCR, alpha, beta, V beta 1; anti-TCR, alpha, beta, V beta 1 antibody;
The TCR, alpha, beta, V beta 1 n/a (Catalog #MBS632259) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TCR, alpha, beta, V beta 1 (T Cell Receptor) reacts with Chicken and may cross-react with other species as described in the data sheet. MyBioSource\'s TCR, alpha, beta, V beta 1 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS).Flow cytometry 1,, Immunohistochemistry (acetone-fixed, frozen sections),, In ovo suppression of the development of TCR-Vb1 cells 5,6,, Immunoprecipitation 1. Researchers should empirically determine the suitability of the TCR, alpha, beta, V beta 1 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TCR, alpha, beta, V beta 1 n/a product has the following accession number(s) (GI #431831552) (NCBI Accession #AGA92551.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the TCR, alpha, beta, V beta 1 (T Cell Receptor) with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Flow Cytometry of chicken PBMC were double stained with T2040-02V3 TCR, alpha, beta, V beta 1 (T Cell Receptor) (PE) and mouse anti-chicken CD3-FITC. Small|lymphocytes were gated and subjected to 2-color analysis on a FACScan (BDIS, San Jose, CA).)
This antibody precipitates a CD3-associated heterodimer of Mr 90kD (two bands of Mr 40kD and 50kD upon reduction) on chicken peripheral blood T cells.1 Deglycosylation of the heterodimer yields two polypeptides of Mr 34kD and 29kD from antibody precipitates. In the chicken, two distinct subpopulations of alpha/beta T cells appear in the thymus subsequent to the appearance of gamma/delta T cells. These subpopulations, originally denoted as TCR2 and TCR3,1-3 arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vbeta families, Vb1 and Vb2, respectively.4 Two-color immunofluorescence has revealed that most of the TCR2+ thymocytes express both CD4 and CD8 antigens. The TCR2+ cells in blood were found to express either CD4 (74±2%) or CD8 (26±4%). TCR2+ cells in the spleen also express either CD4 (37±1%) or CD8 (64±4%). Surprisingly, a relatively large subpopulation of CD8+ cells in the spleen are negative for TCR2.1-3 This observation led to the demonstration that 71±6% of the TCR1(gamma/delta)+ cells in the spleen express the CD8 marker.1-4.
In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TCR, alpha, beta, V beta 1 are readily searchable from our website. Different antibodies against the same target such as TCR, alpha, beta, V beta 1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.