anti-TBC1D10C antibody product blog
Tags: Antibody; Polyclonal Antibody; Carabin; anti-TBC1D10C antibody; TBC1D10C;
The TBC1D10C tbc1d10c (Catalog #MBS150488) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Carabin Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Carabin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC).Carabin antibody can be used for detection of Carabin by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. Researchers should empirically determine the suitability of the TBC1D10C tbc1d10c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TBC1D10C tbc1d10c product has the following accession number(s) (GI #38348348) (NCBI Accession #NP_940919) (Uniprot Accession #Q8IV04). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Carabin Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of Carabin in human spleen tissue lysate with Carabin antibody at (A) 1 and (B) 2 μg/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of Carabin in human spleen tissue with Carabin antibody at 2.5 μg/mL.)
Carabin Antibody: Antigen binding by the T-cell receptor (TCR) is one of the critical first steps in the immune response, triggering a cascade of signaling pathways that ultimately lead to T-cell activation. Screening a yeast two-hybrid screen of a human T-cell cDNA library with calcineurin, a protein phosphatase involved in multiple signaling pathways including T-cell activation, resulted in the identification of Carabin, a member of the TBC1 domain family of proteins, as a calcineurin-binding protein. Unlike other members of the TBC1 domain protein family which are thought to have a role in regulating cell growth and differentiation, further experiments demonstrated that Carabin is part of a negative regulatory loop for the intracellular TCR signaling pathway as well as an inhibitor of the Ras signaling pathway, suggesting that Carabin may also mediate crosstalk between calcineurin and Ras. Carabin antibody does not recognize TBC1D10A or TBC1D10B. Carabin is known to exist in multiple isoforms.
Conjugate: Unconjugated
Immunogen: Carabin antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Carabin.
Buffer: Carabin Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TBC1D10C are readily searchable from our website. Different antibodies against the same target such as TBC1D10C may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.